Coding

Part:BBa_K809717:Design

Designed by: Jianhui Gong   Group: iGEM12_Shenzhen   (2012-09-25)


GAL promoter + ZIM17 + DNase + ADH1 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 1197
    Illegal PstI site found at 2219
    Illegal PstI site found at 2726
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 1197
    Illegal PstI site found at 2219
    Illegal PstI site found at 2726
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 799
    Illegal BglII site found at 1015
    Illegal BglII site found at 1304
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 1197
    Illegal PstI site found at 2219
    Illegal PstI site found at 2726
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 1197
    Illegal PstI site found at 2219
    Illegal PstI site found at 2726
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1429
    Illegal BsaI site found at 1587
    Illegal BsaI site found at 3136
    Illegal BsaI.rc site found at 1251


Design Notes

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Source

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References